Molecular Painting (MP) reagents are non-antibody based, functional biological molecules which naturally associate with the external face of all biological membranes. MP reagents may also be engineered to contain a non-biological (inorganic) component, depending on requirements. Watch this short movie to see how MP works in principal. watch video
Foremost our experience at Anovasia lies with the use of MP reagents together with a large group of viruses known as enveloped viruses and other sub-microscopic biological particles called membrane vesicles or exosomes. All of these types of biological particles can be naturally found in blood or urine and hence provide diagnostic targets. Viruses and particles produced in the laboratory from cell cultures or manufactured on large scale for vaccination or gene delivery/therapy purposes are also equally suited to modifcation via MP.
For DIAGNOSTICS, MP reagents can be mixed into clinical or biological samples to tag, isolate (concentrate/purify), detect and quantify viruses causing infection or exosomes which may signal a certain disease status. watch video The advantage compared to currently used and state-of-the-art technologies is that no antibody technology is required since no prior knowledge of the target agent is necessary. This reduces costs significantly and means that multiple agents can be detected with one single MP reagent.
For VACCINE development, MP reagents can be used for immunomodulation purposes by signaling and targeting vaccine agents to specific immune cell types that are better able to give an immune response e.g. dendritic cells and other antigen presenting cells.
watch video The advantage is that no new vaccine systems need to be developed since current vaccine vectors can be improved with MP reagents without affecting the functions of their surface display. Multiple MP reagents can be reacted onto the surface of the same vector in a dose dependent manner. In this setting, MP can also be used to provide co-stimulatory signals, immunity boosting molecules and/or attenuation of the viral vector.
For GENE DELIVERY and GENE THERAPY, MP reagents can be used to direct or re-direct the pathway of entry for the gene delivery vehicle to a chosen target cell. watch video The advantage is that no new vector systems need to be developed since the current ones can be improved with an MP reagent. A key advantage here is speed and cost since no genetic modification of the cell line producing the vector is required. Additional functions can also be added to existing vectors since the envelope or surface protein of the original vector is not modified or affected by the MP processin any way. For example, a gene therapy viral vector can be modified by an MP reagent with immune-protective functionality to allow longer persistence times in the body. watch video
For RESEARCH purposes, MP reagents can be used to tag and isolate unknown exosomes, membrane vesicles, viruses and infectious agents. For example, enveloped viruses can be tagged with fluorescent MP agents to follow and image disease progression in animal models. watch video This can also be used for rapid concentration of virus or virus vector particles. Anovasia expects this to enable research and diagnostics in third-world countries since no heavy equipment like ultra-centrifuges are required to isolate viruses from samples.
Anovasia Pte. Ltd. holds multiple, exclusive, world-wide liceneses for broad applications of the Molecular Painting technology relating to its use with enveloped viruses, exosomes and related particles.